Gas chromatography and Mass spectroscopy has a various field of applications. The primary area of use is in the separation and analysis of multi component mixtures such as essential oils, hydrocarbons and solvents 12.Gas Chromatography Mass Spectroscopy, a hyphenated system which is a very compatible technique and the most commonly used technique for the identification and quantification of phytocompounds from the herbal medicinal plant origin The unknown phytocomponents in a complex mixture can be determined by the interpretations by matching the spectra with known reference spectra 13.
GC-MS chromatogram of the ethyl acetate extract of Couroupita guianensis (Fig1) shows 17 peaks and has been identified after comparison of the mass spectra with NIST_MSMS, REPLIB and MAINLIB, indicating the presence of 11 phytocompenents 14. The secondary metabolites with their retention time , Molecular formula , Molecular weight and concentration peak area percentage were presented in Table 1. GC-MS analysis of ethyl acetate extract revealed the presence of 11 different compounds namely N-(-)-jasmonoyl-(s)- , Isopropyl amine, Tetracosane, Nonadecane, Octacosane, Squalene, Ergosta-7,22-dien-3-, 4,4,6a,6b,8a,11,11, Alpha-amyrin, Lup-20(29)-en-3-one, Lupeol. The retention time ,molecular weight and the relative percentages of the compounds present in aerial parts of Couroupita guianensis were recorded in Figure 1 and table 1.
The GC-MS spectrum confirmed the presence of 11 major components with the retention time 2.053, 2.12. 31.98, 33.07, 34.13, 35.17, 36.18, 36.18, 36.80, 37.17,38.16,39.15,40.16,44.15, 45.03, 45.33, 46.34 and 46.64 respectively (Table 1). The name of the compound, molecular weight of active principles, molecular formula and structure of the component of the plant material t material were determined 15. N-(-)-jasmonoyl-(s)- is endogenous bioactive jasmonateand it has the hormone triggered activation of jasmonate signaling pathways and structurally similar to coronatine 16. Isopropylamine (2- Aminopropane) is a substances which is used as a food flavouring agent. The pure chemical is corrosive to the eyes, skin, and respiratory tract. Contact with the liquid or vapor can cause severe burns of the eyes and skin; can cause blurred vision or seeing halos around lights, with loss of vision and scarring. Corrosive on ingestion. Swallowing the liquid may cause chemical pneumonitis. Higher exposures can cause pulmonary edema and this can cause death 17. The plant extracts usually occur as a combination of various types of phytoconstituents with different polarities, their separation still remains as big challenge for the process of identification and characterization of phytocompounds from the herbal plants. The preliminary analysis of plant extract may be useful in the detection of bioactive principles and subsequently may lead to the drug discovery and development. HPLC analysis of ethyl acetate extract of aerial parts of Couroupita guianensis were done. The HPLC chromatograph will help as standard chromatogram in future studies, comparing the retention time of isolated compounds with given literatures 18.Figure 2 shows the HPLC analysis of ethyl acetate extract of aerial parts of couroupita guianensis was carried out with the mobile phase methanol: water in the ratio 95:5 gave a total of 25 peaks at retention time 2.171, 2.871, 3.014, 3.637, 4.048, 4.455, 4.580, 4.741, 5.417, 5.896, 6.676, 7.311, 8.563, 9.343, 10.574, 10.826, 12.430, 13.069, 14.607, 15.231, 16.504, 16.957, 19.473, 25.670, 30.669 (Figure : 2). The highest peak was seen at the retention time 3.014 minute 19.